一、細胞分选产品简介

神经末梢元分选是据神经末梢元的功能，将混杂神经末梢元有都具有各不相同优点的几各不相同类群的办法。神经末梢元分选选用的办法是免疫抗体血细胞印迹神经末梢元仪分选和免疫抗体血细胞磁珠神经末梢元分选。此技术水平很广使用于免疫抗体血细胞学、血浆学、肉瘤学和神经末梢海洋生态学中。

二、科学试验原里

免疫抗体系统印迹体血細胞分选是巧用免疫抗体系统印迹体血細胞分选仪，对体血細胞的物理防御、身理、生物制品化学、免疫抗体系统、遗传病、团伙生物制品学遗传性状及特点环境等使用确定或酶联免疫抗体法查重，它可不同放出光的荧光抗弯强度和吸光度将夜光科粒亚群连在一起并可保持单克隆分选，对冗杂样表中的体血細胞使用鉴定费、类型、酶联免疫抗体法和提取。免疫抗体系统磁珠体血細胞分选是把体血細胞用超顺剩磁的 MACS MicroBeads （MACS微磁珠）炎症因子朋友地标注，剩磁标注完后，把以上体血細胞应该通过这个存放强而增强电磁体中的分选柱。分选柱里的栽培基质形成这个高均值电磁体。被剩磁标注的体血細胞延迟在柱里而未被标注的体血細胞则泄露。当分选柱移好电磁体后，延迟柱内的剩磁标注体血細胞就应该被过柱好，这些就非常应该取得标注和未标注的两人体血細胞组份。

三、测试步骤

普鲁士蓝染色生殖细胞分选
1. 取1×108个待监测的细胞系核，加5 ml DPBS洗衣机清洗，室内温度2000 r/min离心力5 min，弃上清，然而用200 μl 0.5% BSA-DPBS重悬细胞系核；
2. 想肿瘤细胞悬液内加入荧光颜料标示的表面抗原，4℃孵育30 min；
3. 将染好的血细胞核用0.5% BSA-DPBS 洗衣机清洗四次，如果用500 μl 0.5% BSA-DPBS重悬，流式的血细胞核仪在线检测；
4. 结局统计数据讲解。
磁珠神经元分选的方法
1. 离心式搜集待提取血神经神经细胞，用极少量PBE孵育液（0.5%BSA、0.08%EDTA，PH7.2），机械泵抽滤去菌及液态体内气态，多方面混悬血神经神经细胞（0.5 ml/1×108血神经神经细胞），填加一抗（10-20 μg/ml终盐浓度），4℃孵育30 min；
2. 用20倍空间PBE洗神经元多次，加个PBE（0.3 ml/1×108神经元）彻底混悬神经元后，放入此类二抗包被的超微磁珠，混匀后摄8~15℃孵育10~15 min；
3. 将分开柱安转入电磁场中，融入0.5 ml PBE，在浮力使用下自动流尽,以预外理分开柱。

Flow cytometry-Cell analysis（流式细胞核术细胞核具体分析）

Flow cytometry is a highly sensitive technology for single cell fluorescent signals measurements. By using specific fluorescent probes, this technology allows simultaneous multiparametric analysis of many thousands of cells per second, enabling researchers to rapidly analyze complex cell populations. 
Cells or particles labeled with fluorescent molecules enter a fluid stream and flow at a constant speed in flow cytometer. When the cells pass through laser focuses along the stream one by one, the fluorescent probes are excited by the laser of specific wavelength and then emit light. The optics collects and transduces fluorescent light to the detector according to its color. Then the fluorescent light is detected, amplified and translated into an electronic signal, which will be processed by signal electronics and sent to a computer for presentation. Information about the cell is recorded and the result can be visually displayed on the computer screen in real time.

Flow cytometry-Cell Sorting（流式组织术组织分选）

Cell sorting is the physical isolation and enrichment of selected cells. Sorting on a flow cytometer is executed just after the standard measuring process. During sorting process the stream is controllably vibrated at a specific frequency to stably break it into droplets. These droplets containing cells with selected values by investigator are then positively or negatively charged more or less and go through a constant electric field. As a result the charged droplets are sent along selected trajectories into vessels (tube or plate), and the uncharged fluid containing non-target cells flow into waste drawer.

When should you use flow cytometric cell sorting?（之类过程中都可以选择免疫印迹组织细胞分选?）

1). To enrich target cell population at a very high purity of 95%-100%.
2). To sort desired cells on the basis of multiple parameter measurements. 
3). To separate cell populations with some low expression antigens on cell surface.
4). To isolate cells according to the accurately quantitative density of specific cell markers. 
5). To acquire one single cell in multi-well plates.
6). To separate cells on the basis of internal constituents or functional staining, e.g. fluorescent hydrolysates. 
7). To detect and sort very rare cells (0.001% or less) from mixed populations.